University faculty face stressors, including multiple roles, institutional pressures, and varying student demands, which may differ by gender and across fields. Using the questionnaire variant of a convergent mixed‐methods design, we examined whether self‐reported work stress and comfort differed by gender and academic field gender stereotype in participants from two universities. Results showed that female faculty reported more quantitative role overload, qualitative role overload, and career development stress than male faculty regardless of field. Qualitative themes centered on institutional barriers and unique pressures for women, although these experiences varied by field. Implications, limitations, and future research for employment counselors are explored.