It is now well established in social science research that scientific knowledge often plays a small role in explaining attitudes toward specific science issues. What typically correlates with these attitudes are individuals’ values and worldviews, such as political ideology, religiosity, and deference to scientific authority (for a review, see National Academies of Sciences Engineering and Medicine, 2017). Although it can be difficult to trace what values consistently predict opinions when science issues are so diverse, knowing which ones typically associate with attitudes toward a variety of scientific issues can benefit scientists, policymakers, and others who strive to effectively communicate the implications of their work to the broader public (Rose et al., 2018). In this research note, we begin to map the similarities and differences between how values correlate with American publics’ views toward three distinct issues—one that is more established (nuclear energy) and two that are more novel (nanotechnology and synthetic biology). We use “publics” in the plural form, given that public audiences are not a uniform group, but rather “almost always subsets of the population,” (National Academies of Sciences Engineering and Medicine, 2016a, p. 132) who hold distinct concerns, values, and degrees of involvement with scientific issues.