Abundant research on the relationship between ideologies and sexual prejudice has not focused on intergroup ideologies such as colorblindness, multiculturalism, and polyculturalism. In three studies with racially and ethnically diverse college and community samples, endorsement of polyculturalism—an ideology focusing on the interactions, influences, and connections among racial and ethnic groups—was associated with lower sexual prejudice (as measured by affective prejudice toward gay men and lesbians, traditional heterosexism, denial of discrimination against homosexuals, and attitudes toward gay men and lesbians). Polyculturalism’s relationship with lower sexual prejudice was mediated by greater openness to criticizing elements of one’s culture that marginalize some groups. Polyculturalism accounted for unique variance in sexual prejudice even when controlling for colorblindness, multiculturalism, social dominance orientation, ethnic identity, conservatism, as well as race and gender. Colorblindness and multiculturalism were not consistently related to lower sexual prejudice. Future work on intergroup ideologies and sexual prejudice is discussed.