The Centers for Disease Control and Prevention (CDC) awards about $300 million to 59 health departments (50 state health departments, 6 directly funded city health departments, and health departments in Puerto Rico, U.S. Virgin Islands, and Washington, D.C.) each year under Program Announcement (PA) 04012 (HIV Prevention Projects for State and Local Health Departments) to deliver comprehensive HIV prevention programs/activities to prevent HIV transmission and acquisition in populations most heavily affected by the epidemic.