The negative and harmful impact that discrimination causes in a human society is that through discrimination human rights and freedoms are violated, foreseen by domestic legislation and international legal acts. The key objective of this research is to analyze the domestic and international legal regulations in the field of discrimination, as well as to address the level of applicability of the law on protection from discrimination in practice. Likewise, in discrimination as a phenomenon, types of unequal treatment such as direct discrimination and indirect discrimination shall be handled. To what extent has Kosovo managed to harmonize domestic legislation with international legislation, as well as how much has it achieved in terms of implementing in practice? The research uses the deduction method, the purpose of which is to elaborate the problem from general to specific arguments. The results of this research indicate the different types of discrimination and the constitutional and legal provisions which guarantee protection from discrimination, as well as the harmonization of domestic legislation with international legislation. Hence, as a precondition when it comes to protection of human rights in terms of non-discrimination, Kosovo must make continuous efforts in the applicability of the legislation in practice.