This study pays attention to within-person fluctuations in meaningful work and its antecedents and consequences. Considering self- and other-oriented dimensions as crucial pathways to meaningful work, effects of daily perceived autonomy support and prosocial impact on one’s meaningful work were examined. A daily diary study was conducted in which 86 nurses from varied hospitals reported their work experiences for 10 consecutive workdays (860 occasions). Results of multilevel modeling showed that both day-level perceived autonomy support and prosocial impact were positively related to day-level meaningful work, which served as the mediator between them and work engagement. Prosocial orientation strengthened the positive relationship between day-level perceived prosocial impact and day-level meaningful work. However, autonomy orientation negatively moderated the effect of day-level perceived autonomy support on day-level meaningful work, suggesting the necessity to distinguish between assisted and asserted autonomy orientation. Our findings illustrate the transient and dynamic nature of meaningful work and provide empirical evidences linking suggested managerial practices to employees’ meaningful work.