The aim of this study was to broadly investigate the role of relationship-, self-, and partner-serving motivation in empathic accuracy in couples’ conflict interactions. To this end, a laboratory study was set up in which couples (n = 172) participated in a conflict interaction task, followed immediately by a video-review task during which they reported their own feelings and thoughts and inferred those of their partner to assess empathic accuracy. We used both trait and state measures of relationship-, self-, and partner-serving motivation, and we experimentally induced these three categories of motivation. Relationship-serving state motivation predicted greater empathic accuracy. In contrast, experimentally induced partner-serving motivation resulted in less empathic accuracy for men. Self-serving motivation was not found to be associated with empathic accuracy, nor were any of the trait measures. These findings underscore the complexity of the association between motivation and empathic accuracy in partners’ conflict interactions.