A better understanding of serological data and risk factors for COVID-19 infection in healthcare workers are especially important in African countries where human resources and health services are more constrained. We reviewed and appraised the evidence of SARS-CoV-2 seroprevalence and its risk factors in healthcare workers in Africa to inform response and preparedness strategies during the SARS CoV-2 pandemic.We followed the PRISMA-ScR guidelines in this scoping review. Databases including PubMed, Embase and preprint servers were searched accordingly from the start of the COVID-19 pandemic to 19th April 2021. Our search yielded twelve peer-reviewed and four pre-print articles comprising data on 9,223 HCWs from eleven countries in Africa. Seroprevalence varied widely and ranged from 0% to 45.1%. Seropositivity was associated with older age, lower education, working as a nurse/non- clinical HCW, or in gynaecology, emergency, outpatient or surgery departments. Asymptomatic rates were high and half of the studies recommended routine testing of HCWs.This scoping review found a varying, but often high SARS-CoV-2 seroprevalence in HCWs in eleven African countries and identified certain risk factors. COVID-19 public health strategies for policy and planning should consider these risk factors and the potential for high seroprevalence among HCWs when prioritizing infection prevention and control measures and vaccine deployment.