The Listening Style Profile (LSP-16) measures people’s self-reported preferences for four listening styles that we habitually use when we listen. Research in this area is important for health care education since listening is a fundamental skill in all the health care professions. The aim of this study was to investigate the factor structure of the LSP-16’s subscales when completed by a group of respondents from a large Australian university. Data from the LSP-16 completed by 860 undergraduate health science students enrolled in eight different courses (response rate of 59%) were analyzed using maximum likelihood confirmatory factor analysis. The model fit indices of the maximum likelihood analysis demonstrate that the resultant models did not fit the data well. One item exhibited model misfit and reliability estimates and factor loadings were modest. The confirmatory factor analysis results did not support the overall latent factor structure of the LSP-16 as initially proposed by its authors. Further work is required to examine the underlying construct validity and other measurement properties of the LSP-16.