This study evaluated the reliability and validity of a Spanish-language translation of the Racial Microaggressions Scale (RMAS; Torres-Harding et al., 2012) and examined whether this Spanish-language RMAS (S-RMAS) could be used as an assessment tool with Spanish-speaking Latinxs. Study participants included 212 adults (mean age 30.6 years) from both university and community samples who identified as Latino/a and who were also predominantly Spanish-speaking or who were bilingual in English and Spanish. Data were collected using both electronic and paper-and-pencil surveys. An exploratory factor analysis produced S-RMAS factors that were theoretically consistent with racial microaggressions literature. The S-RMAS scale demonstrated very good internal consistency, concurrent validity, and convergent validity as evidenced by statistically significant associations with conceptually similar Spanish-language discrimination scales. This is the first study to validate a Spanish-language scale intended to assess Latinx adults’ experiences with racial microaggressions. The implication and limitations of the study were discussed. (PsycInfo Database Record (c) 2021 APA, all rights reserved)