The present study examined how individual differences in motivation to respond without prejudice predict self-reported negative affect and physiological responses to the prejudicial acts of others. One hundred and one White participants were paired with a Black “partner” and together they watched two White men on film having either a pro- or antidiversity discussion. The higher participants were on internal motivation to respond without prejudice, the greater their self-reported negative affect and the more they exhibited distress-related physiological responses during the antidiversity discussion. In contrast, during the prodiversity discussion participants lower in internal motivation to respond without prejudice showed greater physiological distress, but did not self-report more negative affect. These results suggest that only those who have internalized egalitarian goals exhibit the negative emotional responses likely to promote opposition to expressions of intergroup bias; those who lack these goals might instead react against efforts to promote diversity.