Parents are the main socialization agents in the development of emotion regulation (ER). In this study, we evaluated adolescents’ and their respective parents’ perspectives about their use of two ER strategies (cognitive reappraisal and expressive suppression) in daily life. In addition, we evaluated the within-family associations between adolescents’ and their parents’ use of strategies. We controlled for adolescents’ gender and age and the perceived quality of their relationships with their parents (mothers and fathers). The sample consisted of 33 12- to 18-year-old adolescent–father–mother triads, totaling 99 participants. Parents and adolescents reported their use of ER strategies in response to eight random prompts throughout the day, by means of the experience sampling method for 1 week. Participants provided 4082 reports on their momentary experiences. The data were analyzed using multilevel modeling to account for the hierarchical structure of the repeated daily assessments. The significant association between parents’ and adolescents’ use of ER strategies was specific to mother–adolescent dyads. The significant association between adolescents’ and their mothers’ ER strategies varied as a function of the adolescents’ age and the quality of their relationship with their mothers according to adolescents’ reports, but not as a function of adolescent gender. These findings suggest that mothers have a role in their adolescents’ emotion regulation in a developmental period characterized by autonomy from parental guidance.